Part IV: Metabolomics
The Metabolic Fingerprint
Metabolomics is the systematic study of small-molecule metabolites within biological systems. As the downstream products of gene expression and protein activity, metabolites provide the closest readout of an organism's phenotype. Using analytical platforms such as NMR spectroscopy and mass spectrometry, metabolomics enables metabolic profiling, biomarker discovery, and pathway analysis across clinical, environmental, and nutritional applications.
>40,000
Known Metabolites
<1.5 kDa
Molecular Weight Range
NMR + MS
Primary Platforms
Topics in This Part
13. Metabolomics & Metabolic Profiling
Targeted vs untargeted approaches, sample preparation, metabolite identification, and metabolome databases (HMDB, METLIN)
14. NMR & Mass Spectrometry Platforms
¹H and ¹³C NMR metabolomics, LC-MS and GC-MS workflows, ionization modes, and spectral deconvolution
15. Metabolic Pathway & Flux Analysis
Pathway enrichment, metabolic flux analysis (MFA), ¹³C isotope tracing, flux balance analysis, and kinetic modeling
16. Clinical & Environmental Metabolomics
Biomarker discovery, inborn errors of metabolism, pharmacometabolomics, exposomics, and nutritional metabolomics